restriction enzyme digest with ndei Search Results


restriction enzymes bamhi hf  (New England Biolabs)
99
New England Biolabs restriction enzymes bamhi hf
Restriction Enzymes Bamhi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cas9 padh99 plasmid  (Addgene inc)
93
Addgene inc cas9 padh99 plasmid
A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the <t>Cas9</t> plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.
Cas9 Padh99 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cas9 padh99 plasmid - by Bioz Stars, 2026-04
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restriction endonuclease hindiii  (New England Biolabs)
99
New England Biolabs restriction endonuclease hindiii
A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the <t>Cas9</t> plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.
Restriction Endonuclease Hindiii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction endonuclease hindiii - by Bioz Stars, 2026-04
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restriction enzyme digestion  (New England Biolabs)
96
New England Biolabs restriction enzyme digestion
A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the <t>Cas9</t> plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.
Restriction Enzyme Digestion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/restriction enzyme digestion/product/New England Biolabs
Average 96 stars, based on 1 article reviews
restriction enzyme digestion - by Bioz Stars, 2026-04
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hindiii restriction enzyme (10 u/mg dna)  (Bio-Rad)
90
Bio-Rad hindiii restriction enzyme (10 u/mg dna)
A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the <t>Cas9</t> plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.
Hindiii Restriction Enzyme (10 U/Mg Dna), supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hindiii restriction enzyme (10 u/mg dna) - by Bioz Stars, 2026-04
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msci restriction enzyme  (New England Biolabs)
95
New England Biolabs msci restriction enzyme
A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the <t>Cas9</t> plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.
Msci Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/msci restriction enzyme/product/New England Biolabs
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msci restriction enzyme - by Bioz Stars, 2026-04
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restriction enzymes ecorv hf  (New England Biolabs)
96
New England Biolabs restriction enzymes ecorv hf
A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the <t>Cas9</t> plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.
Restriction Enzymes Ecorv Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction enzymes ecorv hf - by Bioz Stars, 2026-04
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restriction enzymes xbai  (New England Biolabs)
99
New England Biolabs restriction enzymes xbai
A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the <t>Cas9</t> plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.
Restriction Enzymes Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction enzymes ecori  (New England Biolabs)
99
New England Biolabs restriction enzymes ecori
A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the <t>Cas9</t> plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.
Restriction Enzymes Ecori, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction enzymes ecori - by Bioz Stars, 2026-04
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restriction enzyme  (New England Biolabs)
95
New England Biolabs restriction enzyme
A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the <t>Cas9</t> plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.
Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/restriction enzyme/product/New England Biolabs
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restriction enzyme - by Bioz Stars, 2026-04
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restriction enzyme bsmai er0031  (Thermo Fisher)
90
Thermo Fisher restriction enzyme bsmai er0031
A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the <t>Cas9</t> plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.
Restriction Enzyme Bsmai Er0031, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction enzyme mspi  (Illumina Inc)
90
Illumina Inc restriction enzyme mspi
A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the <t>Cas9</t> plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.
Restriction Enzyme Mspi, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the Cas9 plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.

Journal: bioRxiv

Article Title: dnaudit + pydnaweb: A lightweight text-based planning and documentation workflow for genetic cloning with automatic verification

doi: 10.1101/2025.05.31.657172

Figure Lengend Snippet: A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the Cas9 plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.

Article Snippet: Finally, Fragment C was co-transformed into C. albicans SC5314 together with the appropriately digested CAS9 pADH99 plasmid (with MssI restriction enzyme; Addgene plasmid # 90979; ) and the donor DNA. dDNAs ( ) were amplified from the template CIp10–γmGFP (Addgene plasmid # 163119, 27) using the oligonucleotides dDNA_ATO1_GFP_Fw and dDNA_ATO1_GFP_Rv as forward and reverse primers, respectively ( Table S1 ).

Techniques: Amplification, Plasmid Preparation, Homologous Recombination

The Cas9 endonuclease is guided by the gRNA to the target site within the ATO1 gene. The gRNA binds to the complementary DNA sequence, allowing Cas9 to introduce a double-strand break (DSB) at that locus (Figure S2). The donor DNA cassette containing the gene encoding GFP facilitates homology-directed repair (HDR), resulting in the insertion of the GFP sequence into the 3’ end of the ATO1 gene.

Journal: bioRxiv

Article Title: dnaudit + pydnaweb: A lightweight text-based planning and documentation workflow for genetic cloning with automatic verification

doi: 10.1101/2025.05.31.657172

Figure Lengend Snippet: The Cas9 endonuclease is guided by the gRNA to the target site within the ATO1 gene. The gRNA binds to the complementary DNA sequence, allowing Cas9 to introduce a double-strand break (DSB) at that locus (Figure S2). The donor DNA cassette containing the gene encoding GFP facilitates homology-directed repair (HDR), resulting in the insertion of the GFP sequence into the 3’ end of the ATO1 gene.

Article Snippet: Finally, Fragment C was co-transformed into C. albicans SC5314 together with the appropriately digested CAS9 pADH99 plasmid (with MssI restriction enzyme; Addgene plasmid # 90979; ) and the donor DNA. dDNAs ( ) were amplified from the template CIp10–γmGFP (Addgene plasmid # 163119, 27) using the oligonucleotides dDNA_ATO1_GFP_Fw and dDNA_ATO1_GFP_Rv as forward and reverse primers, respectively ( Table S1 ).

Techniques: Sequencing, Introduce